ProEasy. Vector for easy construction of E. coli Rec. baculoviruses - 25 ul

ProEasy™ Cat #A10S, #A10L

ProEasy™ is a modified baculovirus genomic DNA which drastically improves upon traditional baculovirus technologies in terms of simplicity of operation and reliable high levels of target protein expression at scale-up of protein production. Due to the simplicity of operation it is also ideal for high throughput applications. Refer to Baculovirus tutorial for simple step-by-step instructions. The improvement is achieved because parental ProEasy™ baculovirus contains a gene knockout that does not allow virus replication under standard conditions which are used for generation and propagation of recombinant baculoviruses (Fig. 1). Therefore, any possibility of background, i.e. contamination with parental nonrecombinant baculovirus has been eliminated. ProEasy™ cannot initiate an infection in insect cells unless it undergoes recombination with a plasmid vector DNA encoding a protein of interest. The commercially available ProEasy™ DNA is also linearized using Bsu36.I restriction endonuclease to favor recombination with the transfer vector. Insertion of a gene of interest occurs into the polyhedrin site of baculovirus DNA under the control of strong polyhedrin promoter. This provides for the highest possible rate of target protein synthesis in the baculovirus system.